Hormonal Induced Spawning of Marine Sea Bass Dicentrarchus labrax L.

Document Type : Original Article

Authors

Department of Animal Production and Fish Resources, Faculty of Agriculture, Suez Canal University, 41522 Ismailia. Egypt.

Abstract

Sea bass brood stocks were caught with average body weight of 2000.0 ± 200 g for males and 3000.0 ± 400 g
for females, from fish farms at Damietta governorate. Brood stocks transported to a private fish farm, situated in Al-
Qantara Gharb, Ismailia governorate, Egypt. A total of 72 brood stocks were divided into four groups with 3 replicates
for each group to evaluate the egg production under the effect of different hormones. Each group consists of 4 males
and 2 females otherwise the sex ratio was two males to one female. Brood stocks were injected when the water
temperature reached 14.8°C during January. The four groups (males and females) were injected as follows; T1: The
control group, brood stocks were injected with physiological saline (0.9% NaCl), T2: injection with 25 ml hCG kg-1
body weight. T3: injection with 50 ml LH-RH kg-1 body weight. T4: injection with two doses: the first dose was injected
at 10 am with 25 ml LH-RH kg-1 body weight and the second dose was injected with 12.5 ml hCG kg-1 body weight
after 24 hrs from the first dose. Fertilization percentage was recorded in group which injected twice with LHRH and
hCG (T4). The injection of LHRH repaired the endocrine disruption that results in the failure of captive fish to undergo
FOM, ovulation and spawning, but hCG could not effect on oocyte development before releasing eggs, but could effect
on latency period and time period of releasing eggs. Therefore, this is an important step towards the

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