Rabbit Semen Characteristics after Passing through Designed Filters

Document Type : Original Article

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Department of Animal Production and Fish resources, Faculty of Agriculture, Suez Canal University, Ismailia, Egypt

Abstract

This study was conducted to improve the quality of rabbit semen by removing dead, immotile and abnormal spermatozoa using five different filtration methods and swim-up technique. Six different semen filtration methods (Sephadex-G15, Albumin, Cotton, Synthetic Fiber, and Sand) and swim-up technique were used. Ten matured rabbit bucks were used for semen collection. Raw and filtered semen samples were evaluated for motility, concentration, and curvilinear velocity by computer assisted sperm analysis (CASA) and membrane integrity by acrosome staining method. Analysis of variance showed significant differences (P≤0.05) due to the combination between filter methods and sperm fractions in progressive motility. The Filtration process improved (P≤0.05) sperm progressive motility than before filtration. Higher sperm motility scores were found in semen fractions two and three (P≤0.05) than that in semen fraction one and in the control sample. High positive correlations were found between the studied semen quality parameters. It could be concluded that most semen quality parameters were improved significantly in all used filters. Sephadex-G15, Sand and Swim-up selection techniques could be more efficient to be practiced routinely in rabbit semen handling. Also, both second and third filtered fractions could be effectively used in artificial insemination (AI) programs.

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